Useful Publications
References appearing in blog articles will be collected here and organized into topics.
Compensation and unmixing
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exaexa/panelbuilder: Panel optimization and unmixing tool for multicolor and spectral cytometry
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Unmixing (David Novo et al.)
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Compensation-induced spread (Mario Roederer)
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Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats (Mario Roederer)
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Compensation in Flow Cytometry (Mario Roederer)
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Improved Compensation in Flow Cytometry by Multi-Variable Optimization (Sugár et al.)
Single color controls: beads or cells
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Ferrer-Font et al. showing side-by-side unmixing with bead or cell controls.
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Konecny et al. using only cell controls for 50-color flow.
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Bhowmick et al. comparing different beads. "It is abundantly clear that bead-based correction produces suboptimal results when applied to cells, compared with cell-based controls applied to cells."
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A practitioner’s view of spectral flow cytometry (Sharma et al.). "We highly recommend the use of cells over beads as single-color controls for spectral unmixing".
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Fluorochrome-dependent specific changes in spectral profiles using different compensation beads or primary cells in full spectrum cytometry (Shevchenko et al.). "While most fluorochromes show the same spectral profile on beads and cells, we demonstrate that specific fluorochromes show a significantly different spectral profile depending on which type of compensation beads is used, and some fluorochromes should be used on cells exclusively."
Antibody titration
- Combinatorial Antibody Titrations (Burns et al.)
Antibody titration
- Combinatorial Antibody Titrations (Burns et al.)
- UWCCC protocol
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Antibody titration
- Combinatorial Antibody Titrations (Burns et al.)
If you have any papers you found useful that aren't covered, please suggest them. New topics are also welcome.