Dealing with multiple autofluorescences
Our samples, more often than not, contain autofluorescence (AF) that varies not only in intensity but also in aspect.
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Nov 255 min read
How to: Run plates on the Cytek Aurora
Today I'm going to share my tips for successfully running lots of samples on the Aurora without having to babysit the machine.
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Nov 1812 min read
Five ways to extract autofluorescence (and store it)
Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.
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Nov 114 min read
Unmixing: should you use a universal negative?
When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".
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Nov 47 min read
Autofluorescence in conventional flow cytometry
Let's take a step back and look at how autofluorescence is handled in conventional flow.
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Oct 311 min read
ClusterID update
Thanks to franpcozar for highlighting an issue with ClusterID . The script was failing when the input clusters were named with...
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Oct 283 min read
Backgating: What is it and why will it help?
Backgating is a simple, useful technique in flow cytometry that can help you figure out where your cells are, and thus optimize your gating
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Oct 214 min read
Autofluorescence: what not to do
Today let’s look at something I think you shouldn’t be doing with AF extraction in spectral flow cytometry.
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Oct 146 min read
How does AutoSpill work and why does this produce better compensation?
There are three key ways in which AutoSpill differs from traditional compensation: Data usage, AF handling and Refinement.
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Oct 14 min read
Thoughts on autofluorescence
In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.
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Sep 243 min read
Panel Design: stress testing
You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.
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Sep 162 min read
Panel Design: Optimizing for cost
Reduce the cost of your panels by focusing on the most expensive reagents.
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Sep 93 min read
Tips & Tricks: Temperature and CCR7 clones
What's the best way to stain for human CCR7?
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Sep 23 min read
Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
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Sep 11 min read
Colibri Cytometry on Substack
I'm going to try out posting Substack as well. That allows people to subscribe for email updates. Check it out if that's your jam....
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Aug 276 min read
Unmixing tips: control clean-up
Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.
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Aug 192 min read
Unmixing tips: using multiple controls
Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.
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Aug 124 min read
Blocking: Tandem breakdown
As much as possible, avoid combining multiple factors that cause tandem breakdown.
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Aug 54 min read
Blocking: CellBlox
CellBlox: a blocking solution for working with NovaFluors
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Jul 294 min read
Blocking: Brilliant Dye interactions
The issue:
Interactions between Brilliant dyes on antibody conjugates.
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