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18 hours ago8 min read
Stabilizing master mixes
How to prepare and store antibody master mixes stably.
222 views2 comments


Feb 36 min read
Simple tips for reducing errors with panel prep
Running large panels consistently is all about error prevention.
315 views3 comments

Jan 206 min read
“Rescuing” GFP
Why do we lose GFP and other fluorescent proteins when we fix and permeabilize cells? How can we stop this?
680 views4 comments


Jan 63 min read
Overnight staining: tetramers
Does overnight staining help for antigen-specific TCR detection with tetramers? Not so much.
222 views0 comments

Dec 16, 20243 min read
Fluorophores for nuclear staining
What's the best type of fluorophore to use for targets that are in the nucleus of the cell?
954 views0 comments

Dec 2, 20248 min read
Dealing with multiple autofluorescences
Our samples, more often than not, contain autofluorescence (AF) that varies not only in intensity but also in aspect.
584 views4 comments


Nov 25, 20245 min read
How to: Run plates on the Cytek Aurora
Today I'm going to share my tips for successfully running lots of samples on the Aurora without having to babysit the machine.
272 views0 comments


Nov 18, 202412 min read
Five ways to extract autofluorescence (and store it)
Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.
336 views0 comments

Nov 11, 20244 min read
Unmixing: should you use a universal negative?
When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".
728 views0 comments

Nov 4, 20247 min read
Autofluorescence in conventional flow cytometry
Let's take a step back and look at how autofluorescence is handled in conventional flow.
821 views2 comments


Oct 31, 20241 min read
ClusterID update
Thanks to franpcozar for highlighting an issue with ClusterID . The script was failing when the input clusters were named with...
31 views0 comments

Oct 28, 20243 min read
Backgating: What is it and why will it help?
Backgating is a simple, useful technique in flow cytometry that can help you figure out where your cells are, and thus optimize your gating
697 views0 comments

Oct 21, 20244 min read
Autofluorescence: what not to do
Today let’s look at something I think you shouldn’t be doing with AF extraction in spectral flow cytometry.
831 views0 comments

Oct 14, 20246 min read
How does AutoSpill work and why does this produce better compensation?
There are three key ways in which AutoSpill differs from traditional compensation: Data usage, AF handling and Refinement.
544 views2 comments

Oct 1, 20244 min read
Thoughts on autofluorescence
In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.
701 views1 comment

Sep 24, 20243 min read
Panel Design: stress testing
You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.
809 views0 comments

Sep 16, 20242 min read
Panel Design: Optimizing for cost
Reduce the cost of your panels by focusing on the most expensive reagents.
531 views2 comments

Sep 9, 20243 min read
Tips & Tricks: Temperature and CCR7 clones
What's the best way to stain for human CCR7?
929 views8 comments

Sep 2, 20243 min read
Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
1,359 views0 comments


Sep 1, 20241 min read
Colibri Cytometry on Substack
I'm going to try out posting Substack as well. That allows people to subscribe for email updates. Check it out if that's your jam....
48 views0 comments
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