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- Sep 2
- 3 min
Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
681 views0 comments
- Sep 1
- 1 min
Colibri Cytometry on Substack
I'm going to try out posting Substack as well. That allows people to subscribe for email updates. Check it out if that's your jam....
21 views0 comments
- Aug 27
- 6 min
Unmixing tips: control clean-up
Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.
438 views2 comments
- Aug 19
- 2 min
Unmixing tips: using multiple controls
Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.
358 views0 comments
- Aug 12
- 4 min
Blocking: Tandem breakdown
As much as possible, avoid combining multiple factors that cause tandem breakdown.
584 views0 comments
- Aug 5
- 4 min
Blocking: CellBlox
CellBlox: a blocking solution for working with NovaFluors
287 views0 comments
- Jul 29
- 4 min
Blocking: Brilliant Dye interactions
The issue:
Interactions between Brilliant dyes on antibody conjugates.
581 views0 comments
- Jul 22
- 3 min
Blocking: Monocyte block
Monocyte Block destroys your cells.
When used intracellularly, Monocyte Block reduces transcription factor staining.
348 views2 comments
- Jul 15
- 6 min
Blocking: Fc block and related reagents
Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.
881 views7 comments
- Jul 10
- 1 min
Flowcytoscript update
The Liston-Dooley lab's flowcytoscript for simplified cytometry data analysis has been updated.
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- Jul 8
- 2 min
Blocking: the value of protein
Today we're looking at the most basic of blocking reagents: generic protein.
597 views2 comments
- Jul 1
- 2 min
Blocking: a cure for all your problems or a cause of all your problems?
In this series, I’m going to review blocking reagents and how to use them for best effect.
1,080 views2 comments
- Jun 24
- 1 min
Colibri resources
If you haven't looked beyond the blog, today is the day to do so. Colibri Cytometry provides other resources, which are highlighted in...
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- Jun 17
- 1 min
How to do different types of flow
An overview of protocols for measuring different modalities by flow cytometry
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- Jun 3
- 4 min
What's that cluster? Part II
If you're interested in this problem, you may wish to get in touch with Ryan Brinkman of Dotmatics regarding the SOULCAP project.
321 views2 comments
- May 27
- 4 min
Data Analysis: Comparing Dimensionality Reductions
In this post, I compared a few dimensionality reduction approaches for visualizing flow cytometry data. I plotted the flow cytometry data...
491 views0 comments
- May 20
- 3 min
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
1,178 views0 comments
- May 13
- 5 min
Data analysis: Scaling your data for dimensionality reduction
Today we're talking about scales. Very boring, very important.
1,289 views0 comments
- May 3
- 4 min
Overnight staining for scRNA-Seq?
The idea? Combine overnight staining with RNA sequencing.
189 views2 comments
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