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The boring details

Which antibodies work for post-fix overnight staining?

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All antibodies listed here have been tested on 2x10^6 (two million) cells using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set. I have not done much testing with the BD equivalent, but it seems to behave similarly. Note that each antibody should be titrated for best results and that the approximate dilutions listed are only a rough guide. The precise dilution factor scales somewhat linearly with the brightness of the fluorophore if the conjugate of a particular clone has been prepared well.

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Additional information can be found on BioLegend's and Thermo's websites, although this is more accurate for PFA fixation.

Human

Mouse

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