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- Sep 2
- 3 min
Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
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- Aug 5
- 4 min
Blocking: CellBlox
CellBlox: a blocking solution for working with NovaFluors
288 views0 comments
- Jul 22
- 3 min
Blocking: Monocyte block
Monocyte Block destroys your cells.
When used intracellularly, Monocyte Block reduces transcription factor staining.
357 views2 comments
- Jul 15
- 6 min
Blocking: Fc block and related reagents
Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.
891 views7 comments
- Jul 8
- 2 min
Blocking: the value of protein
Today we're looking at the most basic of blocking reagents: generic protein.
598 views2 comments
- Jul 1
- 2 min
Blocking: a cure for all your problems or a cause of all your problems?
In this series, I’m going to review blocking reagents and how to use them for best effect.
1,083 views2 comments
- May 20
- 3 min
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
1,180 views0 comments
- Jan 17
- 2 min
Tips & Tricks: Benchmarking controls
Today's topic is benchmarking. This is probably particularly relevant for anyone who manages a shared resource lab with an Aurora. On the...
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- Dec 21, 2023
- 2 min
Tips & Tricks: Stacking antibodies
Improve your signal by using more antibodies, not more antibody. If you have a marker that doesn't stain well, sometimes you can improve...
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- Dec 5, 2023
- 3 min
Tips & Tricks: Dealing with tandems
Tandems, tandems, tandems. We can't seem to avoid them in high parameter flow experiments--they're just too useful. How do we deal with...
1,533 views3 comments
- Nov 28, 2023
- 8 min
Tips & Tricks: Optimizing Unmixing
Today’s post is about how to get the best results with spectral unmixing. Poor quality unmixing is the most common problem I help people...
1,700 views3 comments
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