Unmixing: should you use a universal negative?
When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".
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Oct 283 min read
Backgating: What is it and why will it help?
Backgating is a simple, useful technique in flow cytometry that can help you figure out where your cells are, and thus optimize your gating
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Sep 243 min read
Panel Design: stress testing
You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.
717 views0 comments
Sep 93 min read
Tips & Tricks: Temperature and CCR7 clones
What's the best way to stain for human CCR7?
745 views9 comments
Sep 23 min read
Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
1,138 views0 comments
Aug 276 min read
Unmixing tips: control clean-up
Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.
963 views2 comments
Aug 192 min read
Unmixing tips: using multiple controls
Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.
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Aug 54 min read
Blocking: CellBlox
CellBlox: a blocking solution for working with NovaFluors
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Jul 294 min read
Blocking: Brilliant Dye interactions
The issue:
Interactions between Brilliant dyes on antibody conjugates.
757 views2 comments
Jul 223 min read
Blocking: Monocyte block
Monocyte Block destroys your cells.
When used intracellularly, Monocyte Block reduces transcription factor staining.
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Jul 156 min read
Blocking: Fc block and related reagents
Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.
1,566 views8 comments
Jul 82 min read
Blocking: the value of protein
Today we're looking at the most basic of blocking reagents: generic protein.
698 views2 comments
Jul 12 min read
Blocking: a cure for all your problems or a cause of all your problems?
In this series, I’m going to review blocking reagents and how to use them for best effect.
1,207 views2 comments
May 203 min read
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
1,431 views0 comments
Jan 172 min read
Tips & Tricks: Benchmarking controls
Today's topic is benchmarking. This is probably particularly relevant for anyone who manages a shared resource lab with an Aurora. On the...
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Dec 21, 20232 min read
Tips & Tricks: Stacking antibodies
Improve your signal by using more antibodies, not more antibody. If you have a marker that doesn't stain well, sometimes you can improve...
939 views1 comment
Dec 5, 20233 min read
Tips & Tricks: Dealing with tandems
Tandems, tandems, tandems. We can't seem to avoid them in high parameter flow experiments--they're just too useful. How do we deal with...
1,772 views5 comments
Nov 28, 20238 min read
Tips & Tricks: Optimizing Unmixing
Today’s post is about how to get the best results with spectral unmixing. Poor quality unmixing is the most common problem I help people...
2,400 views4 comments
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