Overnight staining

Cytokine staining: When to stain

How do we deal with marker downregulation during intracellular cytokine staining?

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Overnight staining for scRNA-Seq?

The idea? Combine overnight staining with RNA sequencing.

Optimizing cytokine staining--fixatives

In this post, I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...

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Save money and get better data at the same time!

Watch this talk to learn how leaving your staining for longer can help you get better data for cheaper. Overcome issues of low...

Doing more with less: Fixatives

Which fixative should I use? PFA? A kit? Maybe formalin and dish soap? Answer: It depends! Honestly, it depends a lot on what you're...

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Doing more with less: reducing batch effects

Today I'm going to discuss batch effects, both some thoughts about what causes them and how using overnight staining can help reduce...

Doing more with less: Specificity

Before we get started, it's worth pointing out that while overnight staining is new to flow cytometry, it is pretty commonly used in...

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Doing more with less: viability

Probably the most frequent question I get about overnight staining is along the lines of "won't my cells die?" So today, I'm going to...

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Doing more with less

Today I'm starting a series of posts about how to use overnight staining to improve your flow cytometry. I developed this approach over...

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