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Overnight staining: tetramers
Does overnight staining help for antigen-specific TCR detection with tetramers? Not so much.
Jan 63 min read
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Fluorophores for nuclear staining
What's the best type of fluorophore to use for targets that are in the nucleus of the cell?
Dec 16, 20243 min read
1,087 views
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Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
Sep 2, 20243 min read
1,514 views
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Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
May 20, 20243 min read
1,777 views
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Overnight staining for scRNA-Seq?
The idea? Combine overnight staining with RNA sequencing.
May 3, 20244 min read
318 views
2 comments

Optimizing cytokine staining--fixatives
In this post , I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...
Apr 15, 20242 min read
1,399 views
2 comments


Save money and get better data at the same time!
Watch this talk to learn how leaving your staining for longer can help you get better data for cheaper. Overcome issues of low...
Nov 25, 20231 min read
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Doing more with less: Fixatives
Which fixative should I use? PFA? A kit? Maybe formalin and dish soap? Answer: It depends! Honestly, it depends a lot on what you're...
Oct 15, 20236 min read
8,337 views
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Doing more with less: reducing batch effects
Today I'm going to discuss batch effects, both some thoughts about what causes them and how using overnight staining can help reduce...
Oct 6, 20234 min read
748 views
2 comments

Doing more with less: Specificity
Before we get started, it's worth pointing out that while overnight staining is new to flow cytometry, it is pretty commonly used in...
Oct 3, 20236 min read
3,803 views
4 comments

Doing more with less: viability
Probably the most frequent question I get about overnight staining is along the lines of "won't my cells die?" So today, I'm going to...
Sep 29, 20235 min read
1,496 views
6 comments

Doing more with less
Today I'm starting a series of posts about how to use overnight staining to improve your flow cytometry. I developed this approach over...
Sep 27, 20233 min read
3,109 views
9 comments
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