Apr 297 minHow ToHow to conjugate your own antibodiesWhen we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and
Apr 152 minOvernight stainingOptimizing cytokine staining--fixativesIn this post, I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...
Apr 12 minHow ToOptimizing cytokine staining--IonomycinIntracellular cytokine staining can be challenging to get right. The cells need to be in great condition to withstand the stimulation...
Feb 16 minHow ToHow to test and titrate antibodiesTitration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...
Jun 11, 20231 minHow ToImprove your compensation 100,000-fold!Learn how to use AutoSpill to get practically perfect compensation every time.