How to do different types of flow
An overview of protocols for measuring different modalities by flow cytometry
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- May 20
- 3 min
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
1,180 views0 comments
- Apr 29
- 7 min
How to conjugate your own antibodies
When we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and
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- Apr 15
- 2 min
Optimizing cytokine staining--fixatives
In this post, I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...
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- Apr 1
- 2 min
Optimizing cytokine staining--Ionomycin
Intracellular cytokine staining can be challenging to get right. The cells need to be in great condition to withstand the stimulation...
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- Feb 1
- 6 min
How to test and titrate antibodies
Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...
2,122 views9 comments
- Jun 11, 2023
- 1 min
Improve your compensation 100,000-fold!
Learn how to use AutoSpill to get practically perfect compensation every time.
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