How to: Run plates on the Cytek Aurora
Today I'm going to share my tips for successfully running lots of samples on the Aurora without having to babysit the machine.
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Jun 171 min read
How to do different types of flow
An overview of protocols for measuring different modalities by flow cytometry
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May 203 min read
Cytokine staining: When to stain
How do we deal with marker downregulation during intracellular cytokine staining?
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Apr 297 min read
How to conjugate your own antibodies
When we conjugate an antibody to a fluorophore, we are performing a chemical reaction, creating a covalent bond between the fluorophore and
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Apr 152 min read
Optimizing cytokine staining--fixatives
In this post , I discussed the importance of testing and titrating the stimuli used for intracellular cytokine staining. Today let's look...
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Apr 12 min read
Optimizing cytokine staining--Ionomycin
Intracellular cytokine staining can be challenging to get right. The cells need to be in great condition to withstand the stimulation...
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Feb 16 min read
How to test and titrate antibodies
Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...
2,370 views9 comments
Jun 11, 20231 min read
Improve your compensation 100,000-fold!
Learn how to use AutoSpill to get practically perfect compensation every time.
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