Colibri Cytometry

You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.

Reduce the cost of your panels by focusing on the most expensive reagents.

I suggest starting by designing what I call a backbone for your panel. This is just the basic gating markers that will allow you to...

How to pick the best fluorophores to use in your flow panels.

Once we've decided which markers to include in our panel, the next step is to begin selecting the fluorophores that we want to use in the...

Spread is an inevitable consequence of multicolour fluorescence-based flow cytometry. When an excited fluorophore emits lower energy...

Today let's look at web-tools for understanding fluorophore signatures. These spectral viewer tools can be very useful for designing flow...

A flow panel is all about choices. Our choices with the markers we include and the signals we attach to them via antibodies create a...

Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...

Today's topic is benchmarking. This is probably particularly relevant for anyone who manages a shared resource lab with an Aurora. On the...