Panel Design: Build a backbone
I suggest starting by designing what I call a backbone for your panel. This is just the basic gating markers that will allow you to...
top of page
- Mar 11
- 5 min
Panel Design: selecting fluorophores for spectral flow, part 2
How to pick the best fluorophores to use in your flow panels.
648 views0 comments
- Mar 4
- 5 min
Panel Design: selecting fluorophores for spectral flow, part 1
Once we've decided which markers to include in our panel, the next step is to begin selecting the fluorophores that we want to use in the...
988 views0 comments
- Feb 26
- 5 min
Panel Design: Dealing with spread
Spread is an inevitable consequence of multicolour fluorescence-based flow cytometry. When an excited fluorophore emits lower energy...
1,437 views0 comments
- Feb 19
- 4 min
Panel Design: spectral viewer web-tools
Today let's look at web-tools for understanding fluorophore signatures. These spectral viewer tools can be very useful for designing flow...
547 views0 comments
- Feb 12
- 3 min
Panel Design: Where to start?
A flow panel is all about choices. Our choices with the markers we include and the signals we attach to them via antibodies create a...
1,156 views0 comments
- Feb 1
- 6 min
How to test and titrate antibodies
Titration of antibodies means finding the optimal amount to use in order to maximize signal (specific binding) while minimizing noise...
2,122 views9 comments
- Jan 17
- 2 min
Tips & Tricks: Benchmarking controls
Today's topic is benchmarking. This is probably particularly relevant for anyone who manages a shared resource lab with an Aurora. On the...
626 views0 comments
bottom of page