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Nov 18, 202412 min read
Five ways to extract autofluorescence (and store it)
Let’s start by looking at the autofluorescence extraction methods available to us, their advantages and drawbacks.
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Nov 11, 20244 min read
Unmixing: should you use a universal negative?
When unmixing, we have the option to use either an internal negative population or a completely independent "Universal Negative".
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Oct 14, 20246 min read
How does AutoSpill work and why does this produce better compensation?
There are three key ways in which AutoSpill differs from traditional compensation: Data usage, AF handling and Refinement.
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Aug 27, 20246 min read
Unmixing tips: control clean-up
Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.
1,156 views2 comments

Aug 19, 20242 min read
Unmixing tips: using multiple controls
Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.
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Nov 28, 20238 min read
Tips & Tricks: Optimizing Unmixing
Today’s post is about how to get the best results with spectral unmixing. Poor quality unmixing is the most common problem I help people...
2,980 views3 comments


Jun 11, 20231 min read
Improve your compensation 100,000-fold!
Learn how to use AutoSpill to get practically perfect compensation every time.
812 views2 comments
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