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Welcome to the blog
The content posted here is intended to help and is meant to be as accurate as possible. There will inevitably be mistakes or gaps in the knowledge. This is not peer-reviewed science, but is evidence-based as much as possible. If you see a factual inaccuracy or disagree, add a comment or send in a correction. Also, while some of this content tries to push the boundaries of what we know about how to do flow, it is not intended to claim invention credit. If you feel a reference has been overlooked, please let me know.


Thoughts on autofluorescence
In this series on autofluorescence (AF), I’ll post some musings on how AF affects flow cytometry data and approaches for dealing with it.
Oct 1, 20244 min read
891 views
1 comment


Panel Design: stress testing
You've designed your big flow panel--a great accomplishment! But wait, there's more work to do before you use it.
Sep 24, 20243 min read
947 views
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Panel Design: Optimizing for cost
Reduce the cost of your panels by focusing on the most expensive reagents.
Sep 16, 20242 min read
621 views
7 comments


Tips & Tricks: Temperature and CCR7 clones
What's the best way to stain for human CCR7?
Sep 9, 20243 min read
1,241 views
8 comments


Tips & Tricks: Temperature and time
What are the benefits and drawbacks of staining at higher temperatures?
Sep 2, 20243 min read
1,746 views
0 comments


Colibri Cytometry on Substack
I'm going to try out posting Substack as well. That allows people to subscribe for email updates. Check it out if that's your jam....
Sep 1, 20241 min read
52 views
0 comments


Unmixing tips: control clean-up
Cell-based controls are inherently multi-colour controls. Understanding this is key to getting good unmixing.
Aug 27, 20246 min read
1,524 views
2 comments


Unmixing tips: using multiple controls
Why do we want multiple controls? The most common reason, I expect, is that you'll have a control that you aren't sure will work.
Aug 19, 20242 min read
671 views
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Blocking: Tandem breakdown
As much as possible, avoid combining multiple factors that cause tandem breakdown.
Aug 12, 20244 min read
1,079 views
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Blocking: CellBlox
CellBlox: a blocking solution for working with NovaFluors
Aug 5, 20244 min read
519 views
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Blocking: Brilliant Dye interactions
The issue:
Interactions between Brilliant dyes on antibody conjugates.
Jul 29, 20244 min read
1,610 views
3 comments


Blocking: Monocyte block
Monocyte Block destroys your cells.
When used intracellularly, Monocyte Block reduces transcription factor staining.
Jul 22, 20243 min read
946 views
4 comments


Blocking: Fc block and related reagents
Let's discuss Fc block, which is what most people think of when we talk about "blocking" for flow cytometry.
Jul 15, 20246 min read
3,110 views
9 comments


Flowcytoscript update
The Liston-Dooley lab's flowcytoscript for simplified cytometry data analysis has been updated.
Jul 10, 20241 min read
210 views
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Blocking: the value of protein
Today we're looking at the most basic of blocking reagents: generic protein.
Jul 8, 20242 min read
935 views
2 comments


Blocking: a cure for all your problems or a cause of all your problems?
In this series, I’m going to review blocking reagents and how to use them for best effect.
Jul 1, 20242 min read
1,419 views
2 comments


Colibri resources
If you haven't looked beyond the blog, today is the day to do so. Colibri Cytometry provides other resources, which are highlighted in...
Jun 24, 20241 min read
210 views
0 comments


How to do different types of flow
An overview of protocols for measuring different modalities by flow cytometry
Jun 17, 20241 min read
1,017 views
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What's that cluster? Part II
If you're interested in this problem, you may wish to get in touch with Ryan Brinkman of Dotmatics regarding the SOULCAP project.
Jun 3, 20244 min read
443 views
2 comments


Data Analysis: Comparing Dimensionality Reductions
In this post , I compared a few dimensionality reduction approaches for visualizing flow cytometry data. I plotted the flow cytometry...
May 27, 20244 min read
718 views
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